Evaluation of diversity of Bacilli from chickpea rhizosphere by 16S ARDRA and assessment of their plant-growth-promoting attributes |
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| Authors: | RC Dubey Satyajeet Khare Pankaj Kumar Poonam Dubey DK Maheshwari |
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| Institution: | 1. Department of Botany and Microbiology, Gurukul Kangri Vishwavidyalaya, Haridwar, India.profrcdubey@gmail.com;3. Department of Botany and Microbiology, Gurukul Kangri Vishwavidyalaya, Haridwar, India. |
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| Abstract: | A total of eight motile, aerobic, Gram-positive and straight rod-shaped, endospore forming Bacillus spp. were isolated from rhizosphere of chickpea plants collected from different agricultural fields. Phylogeny of isolates was studied by partial sequencing of 16S rDNA and comparative analysis of the sequence data confirmed that the isolates belong to distinct phylogenetic lineage corresponding to Bacillus. Phenotyping clusters correlate with ARDRA pattern and showed resemblance to partial 16S rDNA sequencing. Bacillus spp. BSK5 and Bacillus subtilis BSK17 were the most potent strains for having plant-growth-promoting attributes. These two strains solubilised inorganic phosphate, produced Indole acetic acid, siderophore, Hydrocyanic acid and secreted extracellular chitinase and mild β-1,3-glucanase which antagonised and caused mycelial deformities in two phytopathogens Macrophomina phaseolina and Fusarium oxysporum in dual culture and by culture filtrate. |
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| Keywords: | Bacillus spp chickpea 16S rDNA ARDRA M phaseolina F oxysporum |
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