Detection of begomovirus associated with okra leaf curl disease |
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| Authors: | Sayed Sartaj Sohrab Zeenat Mirza Sajjad Karim Debashis Rana Adel M Abuzenadah Adeel G Chaudhary |
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| Institution: | 1. King Fahd Medical Research Centre, King Abdulaziz University , Jeddah , Saudi Arabia;2. Division of Biotechnology , J K Agri-Genetics Ltd , Hyderabad, India ssohrab@kau.edu.sa;4. King Fahd Medical Research Centre, King Abdulaziz University , Jeddah , Saudi Arabia;5. Centre of Excellence in Genomic Medicine Research , King Abdulaziz University , Jeddah , Saudi Arabia;6. Division of Biotechnology , J K Agri-Genetics Ltd , Hyderabad, India |
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| Abstract: | Leaf curl and yellow vein mosaic viral disease is the major constraint on okra (Abelmoschus esculentus L.) production in India. Amplified fragment sequence of DNA-β showed highest similarity of 91.7% with Bhendi yellow vein mosaic virus-Tamil Nadu (AJ308425, NC_003405) and lowest similarity of 48.5% with OKLCV (NC_004093), whereas coat protein specific amplified sequence showed highest homology with isolate of Madurai, Haryana, Ludhiana and lowest homology of 92% with Mesta yellow vein mosaic Bahraich virus (MYVMBV) (EU360303). The results obtained in the present study confirm that both the viral diseases of okra reported in southern India are caused by a begomovirus associated with DNA-β in which the plants show leaf curl symptoms and never develops yellow vein mosaic and those plants which show yellow vein mosaic, never develops leaf curl symptoms even in the same rows and field. The okra leaf curl is an emerging virus disease in India. |
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| Keywords: | begomovirus okra OKLCV DNA-β coat protein gene |
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