In vitro antifungal activity and mode of action of selected polyphenolic antioxidants on Botrytis cinerea |
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Authors: | Shutian Tao Shaoling Zhang Rong Tsao Marie Thérèse Charles Raymond Yang |
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Affiliation: | 1. College of Horticulture, Nanjing Agricultural University , 1 Weigang, Nanjing, 210095, Jiangsu Province, P.R. China;2. Horticultural Research and Development Centre, Agriculture and Agri-Food Canada , 430 Gouin Blvd., St-Jean-sur-Richelieu, J3B 3E6, Quebec, Canada;3. College of Horticulture, Nanjing Agricultural University , 1 Weigang, Nanjing, 210095, Jiangsu Province, P.R. China;4. Food Research Program, Agriculture and Agri-Food Canada , 93 Stone Road West, Guelph, N1G 5C9, Ontario, Canada;5. Horticultural Research and Development Centre, Agriculture and Agri-Food Canada , 430 Gouin Blvd., St-Jean-sur-Richelieu, J3B 3E6, Quebec, Canada |
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Abstract: | Six selected antioxidants (catechin, quercetin-3-galactoside, cyanidin-3-glucoside, pelargonidin-3-glucoside, ellagic and gallic acids) were evaluated in vitro for their antifungal activities and mode of action on Botrytis cinerea Pers., one of the most important pathogens of strawberries. Inhibitory effects were found for all the tested antioxidants, but varied at different fungal developmental stages. Catechin and quercetin-3-galactoside showed linear inhibitory effects on germ tube elongation, with the highest suppression ratios of 54.8% and 58.8% respectively. No significant effect was found on spore germination between treatments and control. Gallic acid showed very strong and linear inhibition on spore germination (r = ?0.95), but the effect diminished after spore germination. Cyanidin-3-glucoside and pelargonidin-3-glucoside provided effective control on the fungi as concentrations increased. The arresting effect of ellagic acid on development of B. cinerea was quadratic. Ellagic acid inhibited germ tube elongation and mycelial growth at its highest and lowest concentrations, while no effects were observed at its medium concentration used in this study. |
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Keywords: | Botrytis cinerea ellagic acid gallic acid anthocyanin catechin flavonol |
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