In vitro effect of fermented maize slurry and ethanol extracts of five plants against Sclerotium rolfsii Sacc. of tomato (Lycopersicon esculentum Mill.) |
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Authors: | EA Adesegun AO Akintokun OS Adebayo MO Bankole |
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Institution: | 1. Spices Research Programme, National Horticultural Research Institute , Ibadan , Nigeria nihortinfo@yahoo.com nihortinfo@yahoo.com;3. Department of Microbiology , Federal University of Agriculture , Abeokuta , Nigeria;4. Spices Research Programme, National Horticultural Research Institute , Ibadan , Nigeria |
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Abstract: | Sclerotium rolfsii Sacc. is an important fungal pathogen affecting the production of tomato (Lycopersicon esculentum Mill.). Conventional control methods using chemicals are expensive, which constitute environmental hazards. This has necessitated the search for alternatives in botanicals. Ethanol and supernatant solution of fermented maize slurry extracts from Ocimum gratissimum, Cymbopogon citratus, Xylopia aethiopica, Aframomum melegueta and Allium sativum were evaluated. In vitro test was carried out at 1, 2, 3, 4 and 5% concentrations against the pathogen. Mycelial growth was significantly reduced in the following descending order: O. gratissimum?>?A. melegueta?>?A. sativum?>?X. aethiopica?>?C. citratus. Supernatant solution extracts of O. gratissimum and A. melegueta at 5% concentration gave highest mycelial reduction (0.00?mm), respectively, while O. gratissimum ethanol extract recorded highest mycelial reduction (0.23?mm) among the ethanol extracts. Supernatant solution of fermented maize slurry extracts of O. gratissimum and A. melegueta at 5% concentration was more effective in reducing mycelial growth of S. rolfsii. |
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Keywords: | antifungal botanicals tomato Sclerotium rolfsii extraction solvent |
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