Real-time PCR detection and quantification of Fusarium poae,F. graminearum,F. sporotrichioides and F. langsethiae in cereal grains in Finland and Russia |
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| Authors: | T Yli-Mattila S Paavanen-Huhtala M Jestoi P Parikka V Hietaniemi T Gagkaeva |
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| Institution: | 1. Laboratory of Plant Physiology and Molecular Biology, Department of Biology , University of Turku , tymat@utu.fi;3. Laboratory of Plant Physiology and Molecular Biology, Department of Biology , University of Turku ,;4. Finnish Food Safety Authority (EVIRA), Department of Animal Diseases and Food Research, Unit of Chemistry and Toxicology , 4MTT Afrifood Research Finland, Laboratories , Jokioinen, Finland;5. Plant Production Research/Plant Protection, MTT Agrifood Research Finland , Jokioinen;6. MTT Chemistry Laboratory, MTT Agrifood Research Finland , Jokioinen, Finland;7. Department of Mycology and Phytopathology , All-Russian Institute of Plant Protection (VIZR) , St Petersburg-Pushkin, Russia |
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| Abstract: | Abstract TaqMan real-time quantitative PCR assays were developed for the accurate detection and quantification of DNA from Fusarium poae and F. graminearum species, which are able to produce trichothecenes. These and other PCR assays were used for the quantification of trichothecene-producing Fusarium fungi in cereal grains. A correlation was found between the levels of F. poae DNA and nivalenol and enniatins in barley and between the levels of F. graminearum DNA and deoxynivalenol in oats. The correlations between F. poae DNA and nivalenol and F. graminearum DNA and deoxynivalenol levels were higher than those between these mycotoxins and morphologically determined F. poae and F. graminearum/F. culmorum contamination levels. The use of F. poae specific primers and probe together with F. sporotrichioides/F. langsethiae specific primers and probe in a multiplex qPCR assay yielded results in accordance with those obtained using these primers and probes separately. |
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| Keywords: | Barley deoxynivalenol enniatins nivalenol oats TaqMan PCR |
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