Crude honey bee venom and Bacillus thuringiensis L. Cry1c toxin in controlling wax moth Achroia grisella F. (Lepidoptera: Pyralidae) |
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| Authors: | Montasir Omer Mahgoub Wei Hong Lau Dzolkhifli Bin Omar |
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| Institution: | 1. Faculty of Agriculture, Department of Plant Protection, University Putra Malaysia, Serdang, Malaysiamont132@gmail.com;3. Faculty of Agriculture, Department of Plant Protection, University Putra Malaysia, Serdang, Malaysia |
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| Abstract: | Achroia grisella, is a noxious pest of honey bee hives. In this study the toxicity of honey bee venom and bacterial Cry1C toxin of Bacillus thuringiensis on A. grisella were assessed. In addition, the synergistic interaction between BT Cry1c and crude honey bee venom was determined. The combination of HBV and Cry1C increased the activity for both toxins. Therefore, further analysis was carried out to assess the synergistic interaction of each compound assay separately and in mixture. The results showed LC50 value of 1.105 μg/μl for HBV and LC50 value of 0.201 μg/μl for Cry1C. The combination bioassay result showed an LC50 value of 0.549 μg/μl. The combined calculated LC50 is containing lower concentration of the component toxins, this result proved that the toxicity was enhanced due to the combination. A chi square value of 39.93and relative synergistic factor ratio of 1.14 confirmed that a synergic interaction was achieved in combination. |
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| Keywords: | crude honey bee venom Cry1C synergistic interaction |
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