Development and comparison of ELISA and PCR methods for the early detection of Ganoderma disease of coconut |
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| Authors: | M. Karthikeyan R. Bhaskaran K. Radhika S. Mathiyazhagan V. Jayakumar R. Sandosskumar |
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| Affiliation: | 1. AC &2. RI TNAU Madurai , Indiakarthipath@rediffmail.com;4. Coconut Research Station , Veppankulam, 614 906, Tamil Nadu, India;5. Faculty of Agriculture , Annamalai University , Annamalainagar, 608 002, Tamil Nadu, India;6. RI TNAU Madurai , India;7. Central Agricultural Research Institute (CARI) , Port Blair, 744 101, Andaman Nicobar Islands, India |
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| Abstract: | Abstract Molecular diagnosis, chemo-diagnosis and physiological parameter have been applied for detecting the Ganoderma disease of coconut. Polyclonal antibodies (PAbs) raised against mycelial protein of Ganoderma, specific mycelial protein (62 kDa) of Ganoderma isolates and basidiocarp protein of Ganoderma were used for detection. All the PAbs could detect Ganoderma in diseased coconut root tissues in early stage of the disease before symptom expression by indirect – ELISA at the antiserum dilution of 1:1000 for mycelial protein, 1:700 for specific protein and 1:3000 for basidiocarp protein. Low cross reactions were observed with saprophytic fungi occurring in coconut roots and also with other basidiomycetous fungi. For polymerase chain reaction tests, the primer was generated from the internal transcribed spacer region one (ITS 1) of rDNA of Ganoderma, which produced a PCR product of 167 bp in size. Utility of this method was confirmed at the field level. |
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| Keywords: | Polyclonal antibodies Ganoderma ELISA basidiomycetous internal transcribed spacer polymerase chain reaction |
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