Morphological and molecular identification of some closely related Pythium species in Egypt |
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| Authors: | Youssuf AMH Gherbawy Dr Hani MA Abdelzaher Jochen Meens Hesham El-Hariry |
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| Affiliation: | 1. Institute of Microbiology, Hannover University , Schneiderberg, Hannover, Germany Youssufgherbawy@yahoo.com;3. Botany Department , Faculty of Science, El-Minia University , El-Minia, Egypt;4. Institute of Microbiology, Hannover University , Schneiderberg, Hannover, Germany |
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| Abstract: | Twelve isolates of Pythium species (P. aphanidermatum, P. deliense, P. ultimum var. ultimum and P. ultimum var. sporangiiferum) from different hosts were compared from morphological, pathological and molecular viewpoints. Minimum, optimum and maximum temperatures of P. aphanidermatum and P. deliense were similar while those of P. ultimum var. ultimum and P. ultimum var. sporangiiferum were also similar. All tested isolates were highly virulent against cucumber seedlings with 100% damping-off. RAPD data using three different primers revealed that strains of P. ultimum var. ultimum and P. ultimum var. sporangiiferum are distinct from each other. This data can be used to separate those species from P. aphanidermatum and P. deliense. In contrast, RAPD data cannot be used to separate P. aphanidermatum and P. deliense. Sequence analysis of the ribosomal DNA internal transcribed spacers (ITS) was used to establish phylogenetic relationships among the tested isolates. |
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| Keywords: | Pathogenicity RAPD-PCR ITS regions |
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