Xanthomonas oryzae pv. oryzae infection triggers accumulation of phenolics,defense-related enzymes and thaumatin-like proteins in rice leaves |
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| Authors: | R. Velazhahan J. Jayaraj Salaheddin Khabbaz R. Sandoss Kumar S. Muthukrishnan |
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| Affiliation: | 1. Department of Plant Pathology , Centre for Plant Protection Studies, Tamil Nadu Agricultural University , Coimbatore, Tamil Nadu, India velazhahan@hotmail.com;3. Department of Biochemistry , Kansas State University , Manhattan, Kansas, USA;4. Department of Plant Pathology , Centre for Plant Protection Studies, Tamil Nadu Agricultural University , Coimbatore, Tamil Nadu, India |
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| Abstract: | Abstract The effect of Xanthomonas oryzae pv. oryzae infection on induction of phenylalanine ammonia-lyase (PAL), peroxidase (PO), phenolics and thaumatin-like proteins (TLPs) in rice was studied. PAL activity increased significantly one day after inoculation with X. o. pv. oryzae and the maximum enzyme activity was observed two days after inoculation. The phenolic content in rice leaves increased significantly one day after inoculation and the maximum accumulation of phenols was observed two days after inoculation. Significant increase in peroxidase activity was observed in rice leaves one day after inoculation with X. o. pv. oryzae. Isozyme analysis indicated that three peroxidase isozymes (PO-1, PO-2 and PO-3) were induced after inoculation with X. o. pv. oryzae. Immunoblot analysis of protein extracts from control and pathogen inoculated rice plants revealed the induced accumulation of 16 and 24 kDa TLPs in rice leaves in response to X. o. pv. oryzae infection. TLP mRNA accumulation was induced strongly in rice leaves in response to infection by X. o. pv. oryzae. |
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| Keywords: | Oryza sativa bacterial leaf blight phenolics peroxidase phenylalanine ammonia-lyase thaumatin-like proteins |
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