Cytological characterization of NOR in the bivalent of Saccharomyces cerevisiae |
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| Authors: | T Kuroiwa S Miyamura S Kawano M Hizume A Tho-E I Miyakawa N Sando |
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| Affiliation: | 1. CNRS, UMR 7144, Adaptation et Diversité en Milieu Marin, Station Biologique de Roscoff, 29680 Roscoff, France;2. Sorbonne Universités, UPMC Univ Paris 06, Station Biologique de Roscoff, 29680 Roscoff, France;3. CNRS/MNHN, UMR 7221, Evolution des Régulations Endocriniennes, MNHN, 7 Rue Cuvier, 75231 Paris Cedex 05, France;1. Laboratory Network for Environmental Monitoring (LRSE), Department of Biology, University of Oran 1 Ahmed Ben Bella, BP 1524 El M''naouer, 31000 Oran, Algeria;2. Department of Applied Molecular Genetics, Faculty of Natural and Life Sciences, University of Science and Technology Mohamed Boudiaf USTO/MB, BP 1505 El Menaouar, 31036 Oran, Algeria;3. Central Environmental Laboratory (GR-CEL), Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland;4. Faculty of Geosciences and Environment, University of Lausanne, 1015 Lausanne, Switzerland;1. Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, Mexico City, Mexico;2. Instituto Nacional de Cancerología, Mexico City, Mexico |
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| Abstract: | We cytologically characterized the nucleolar organizer region (NOR) on the bivalent in the yeast Saccharomyces cerevisiae. We used staining with 4'-6-diamidino-2-phenylindole (DAPI), chromomycin A3, and silver nitrate and in situ hybridization technique and utilized a video-intensified microscope system with an ultra-high-sensitive video camera. The results showed that of 16 bivalents of S. cerevisiae, the longest was a recognizable nucleolar chromosome which has an annular and synaptonemal complexless NOR in its submedian portion. The NOR was comprised of 2.65 X 10(9) D DNA which corresponded to 118 copies per haploid of rDNA repeating units. This evidence is discussed in terms of the possible participation of the annular NOR in suppressing the meiotic recombination of the rDNA gene clusters. |
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