Metabolic engineering of Corynebacterium glutamicum for production of sunscreen shinorine |
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| Authors: | Yota Tsuge Hideo Kawaguchi Shogo Yamamoto Yoshiko Nishigami Masahiro Sota Chiaki Ogino |
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| Affiliation: | 1. Graduate School of Natural Science and Technology, Kanazawa University , Kanazawa, Japan;2. Institute for Frontier Science Initiative, Kanazawa University , Kanazawa, Japan;3. Graduate School of Science, Technology and Innovation, Kobe University , Kobe, Japan;4. Nagase R&5. D Center, Nagase &6. Co., Ltd ., Kobe, Japan;7. Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University , Kobe, Japan |
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| Abstract: | Ultraviolet-absorbing chemicals are useful in cosmetics and skin care to prevent UV-induced skin damage. We demonstrate here that heterologous production of shinorine, which shows broad absorption maxima in the UV-A and UV-B region. A shinorine producing Corynebacterium glutamicum strain was constructed by expressing four genes from Actinosynnema mirum DSM 43827, which are responsible for the biosynthesis of shinorine from sedoheptulose-7-phosphate in the pentose phosphate pathway. Deletion of transaldolase encoding gene improved shinorine production by 5.2-fold. Among the other genes in pentose phosphate pathway, overexpression of 6-phosphogluconate dehydrogenase encoding gene further increased shinorine production by 60% (19.1 mg/L). The genetic engineering of the pentose phosphate pathway in C. glutamicum improved shinorine production by 8.3-fold in total, and could be applied to produce the other chemicals derived from sedoheptulose-7-phosphate. |
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| Keywords: | Corynebacterium glutamicum metabolic engineering shinorine pentose phosphate pathway |
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