Signal-anchor sequences are an essential factor for the Golgi-plasma membrane localization of type II membrane proteins |
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| Authors: | Tatsuki Kikegawa Takuya Yamaguchi Ryohei Nambu Kenji Etchuya Masami Ikeda |
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| Affiliation: | 1. Department of Electronics, Graduate School of Science and Technology, Meiji University, Kanagawa, Japan;2. Molecular Neurobiology Research Group, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki, Japan;3. Department of Electronics and Bioinformatics, School of Science and Technology, Meiji University, Kanagawa, Japan;4. Artificial Intelligence Research Center (AIRC), National Institute of Advanced Industrial Science and Technology (AIST), Tokyo, Japan |
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| Abstract: | Despite studies of the mechanism underlying the intracellular localization of membrane proteins, the specific mechanisms by which each membrane protein localizes to the endoplasmic reticulum, Golgi apparatus, and plasma membrane in the secretory pathway are unclear. In this study, a discriminant analysis of endoplasmic reticulum, Golgi apparatus and plasma membrane-localized type II membrane proteins was performed using a position-specific scoring matrix derived from the amino acid propensity of the sequences around signal-anchors. The possibility that the sequence around the signal-anchor is a factor for identifying each localization group was evaluated. The discrimination accuracy between the Golgi apparatus and plasma membrane-localized type II membrane proteins was as high as 90%, indicating that, in addition to other factors, the sequence around signal-anchor is an essential component of the selection mechanism for the Golgi and plasma membrane localization. These results may improve the use of membrane proteins for drug delivery and therapeutic applications. |
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| Keywords: | Subcellular localization signal-anchor type II membrane protein hydropathy profile position-specific scoring matrix |
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