Inhibition of miR-21-5p suppresses high glucose-induced proliferation and angiogenesis of human retinal microvascular endothelial cells by the regulation of AKT and ERK pathways via maspin |
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Authors: | Feng Qiu Huijuan Tong Yawen Wang Jun Tao Hailin Wang |
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Institution: | 1. Department of Ophthalmology, The First Affiliated Hospital of China Medical University, Shenyang, People’s Republic of China;2. Department of Ophthalmology, Shenyang Fourth People’s Hospital, Shenyang, People’s Republic of China;3. Department of Nursing, Shenyang Medical College, Shenyang, People’s Republic of China;4. Department of Ophthalmology, Shenyang Fourth People’s Hospital, Shenyang, People’s Republic of China |
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Abstract: | The aim of the present study is to investigate the role of miR-21-5p in angiogenesis of human retinal microvascular endothelial cells (HRMECs). HRMECs were incubated with 5 mM glucose, 30 mM glucose or 30 mM mannitol for 24 h, 48 h or 72 h. Then, HRMECs exposed to 30 mM glucose were transfected with miR-21-5p inhibitor. We found that high glucose increased the expression of miR-21-5p, VEGF, VEGFR2 and cell proliferation activity. Inhibition of miR-21-5p reduced high glucose-induced proliferation, migration, tube formation of HRMECs, and reversed the decreased expression of maspin as well as the abnormal activation of PI3K/AKT and ERK pathways. Down-regulation of maspin by siRNA significantly increased the activities of PI3K/AKT and ERK pathways. In conclusion, inhibition of miR-21-5p could suppress high glucose-induced proliferation and angiogenesis of HRMECs, and these effects may partly dependent on the regulation of PI3K/AKT and ERK pathways via its target protein maspin. |
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Keywords: | miR-21-5p proliferative diabetic retinopathy human retinal microvascular endothelial cells proliferation angiogenesis |
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