Xylanase and feruloyl esterase from actinomycetes cultures could enhance sugarcane bagasse hydrolysis in the production of fermentable sugars |
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| Authors: | Nanik Rahmani Prihardi Kahar Puspita Lisdiyanti Euis Hermiati Jaemin Lee Yopi |
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| Institution: | 1. Research Center for Biotechnology, Indonesian Institute of Sciences, Cibinong Science Center, Cibinong, Indonesia;2. Department of Chemical and Engineering, Graduate School of Engineering, Kobe University, Kobe, Japan;3. Research Center for Biomaterial, Indonesian Institute of Sciences, Cibinong Science Center, Cibinong, Indonesia |
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| Abstract: | The addition of enzymes that are capable of degrading hemicellulose has a potential to reduce the need for commercial enzymes during biomass hydrolysis in the production of fermentable sugars. In this study, a high xylanase producing actinomycete strain (Kitasatospora sp. ID06-480) and the first ethyl ferulate producing actinomycete strain (Nonomuraea sp. ID06-094) were selected from 797 rare actinomycetes, respectively, which were isolated in Indonesia. The addition (30%, v/v) of a crude enzyme supernatant from the selected strains in sugarcane bagasse hydrolysis with low-level loading (1 FPU/g-biomass) of Cellic® CTec2 enhanced both the released amount of glucose and reducing sugars. When the reaction with Ctec2 was combined with crude enzymes containing either xylanase or feruloyl esterase, high conversion yield of glucose from cellulose at 60.5% could be achieved after 72 h-saccharification. |
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| Keywords: | Actinomycetes xylanase feruloyl esterase sugarcane bagasse fermentable sugars |
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