Direct bioethanol production from brown macroalgae by co-culture of two engineered Saccharomyces cerevisiae strains |
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| Authors: | Yusuke Sasaki Toshiyuki Takagi Keisuke Motone Toshiyuki Shibata Kouichi Kuroda |
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| Institution: | 1. Graduate School of Advanced Integrated Studies in Human Survivability, Kyoto University , Kyoto, Japan;2. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University , Kyoto, Japan;3. CREST, JST , Kyoto, Japan;4. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University , Kyoto, Japan;5. CREST, JST , Kyoto, Japan;6. Japan Society for the Promotion of Science , Kyoto, Japan;7. CREST, JST , Kyoto, Japan;8. Department of Life Sciences, Graduate School of Bioresources, Mie University , Mie, Japan |
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| Abstract: | A co-culture platform for bioethanol production from brown macroalgae was developed, consisting of two types of engineered Saccharomyces cerevisiae strains; alginate- and mannitol-assimilating yeast (AM1), and cellulase-displaying yeast (CDY). When the 5% (w/v) brown macroalgae Ecklonia kurome was used as the sole carbon source for this system, 2.1 g/L of ethanol was produced, along with simultaneous consumption of alginate, mannitol, and glucans. |
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| Keywords: | Bioethanol brown macroalgae alginate mannitol Saccharomyces cerevisiae |
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