黄山药愈伤组织诱导与分化

采用黄山药野生植株作为外植体，试验了不同激素处理对黄山药愈伤组织的诱导、分化影响，结果表明：不同的外植体的诱导率差别较大，叶片的诱导率最高，最高达到85．7％，茎段的诱导率较低，平均诱导率仅10％左右。以叶片作为外植体诱导愈伤组织的最佳培养基配方为MS 2，4-D2．0mg／L 6-BA2．5mg／L；愈伤组织分化生芽的最佳配方为MS BA1．0mg／L NAA0．5mg／L 蔗糖2％ pH6．4；愈伤组织分化生根的最佳配方诱MS BA1．0mg／L NAA0．1mg／L 蔗糖3％ pH6．80。

Callus Induction and Differentiation of Dioscorea panthaica

The Dioscorea panthaica callus induction and differentiation were studied with different hormones when it used the wild Dioscorea panthaica as explant. The result showed: Different explants would bring great difference of the induction rate, the induction rate of the blade was the highest, up to 85 .7%, the induction rate of the stem was relatively low, only about 10% equally. When it used blade as explant, the best callus induction formula was 2.0ml/L 2,4-D and 2.5ml/L 6-BA were added in the MS culture medium, the best bud differentiation formula was 0.5ml/L NAA and 1.0ml/L BA were added in the MS medium, with 2% sugar at pH6.4. And the root differentiation rate was highest when 0.1ml/L NAA and 1.0ml/L BA were added in it,with 3% sugar at pH6.8.