Movement of the 3'-end of 16 S RNA towards S21 during activation of 30 S ribosomal subunits |
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| Authors: | O.W. Odom G. Stöffler B. Hardesty |
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| Affiliation: | 1. The Clayton Foundation Biochemical Institute, University of Texas, Austin, TX 78712, USA;2. Max-PlanckInstitut für Molekulare Genetik, Abteilung Wittmann, Ihnestrasse 63-73, 1000 Berlin 33 (Dahlem), Germany |
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| Abstract: | Fluorescence techniques were used to study conformational changes that occur in inactive E. coli 30 S ribosomal subunits during activation by heating in 12 mM Mg2+. Activation is associated with movement of a fluorophore on the 3'-end of 16 S RNA into a less polar environment and towards a probe on the cysteine thiol of ribosomal protein S21. The conformational change causes an apparent decrease in distance between the probes from 59 to 52 A as determined by non-radiative energy transfer. |
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| Keywords: | 30 S subunit Ribosome activation Protein S21 3'-end 16 S RNA Fluorescence 30 S conformation CPM, 3-(4-maleimidylphenyl)-4-methyl-7-diethylaminocoumarin FTS, fluorescein 5'-thiosemicarbazide DCCH, 7-diethylaminocoumarin-3-carbohydrazide 30 S(-S21), 30 S subunits from which protein S21 has been removed Hepes, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid CPM-S21, S21 labeled at its sulfhydryl group with CPM FTS-16 S RNA and DCCH-16 S RNA, 16 S RNA labeled at its 3'-end with FTS or DCCH |
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