鹅源新城疫病毒血凝素-神经氨酸酶基因的序列分析

Six isolates of Newcastle disease virus were derived from south and east China regions during the disease outbreaks called "avian paramyxovirus infection of geese" or "geese paramyxovirus infection",and partial sequence analysis of hemagglutinin-neuraminidase(HN) gene was carried out to identify the genetic characteristics of these goose isolatesA 1905 nucleotide portion of HN gene of each of the 6 isolates was sequenced,the results revealed that the coding region of their HN genes are all 1716 nucleotides in length,which can encode 571 amino acid residues aloneThe coding region is followed by a noncoding sequence of 189 nucleotidesThough they diverged only 08%-37% from each other in the nucleotide sequences of coding region,they differed by 175%-179% to F48E8, a standard challenge strain of chicken originCysteine residues are well conserved throughout the amino acid sequences,while the number of the potential glycosylation sites varys from 4 to 6Residue positions Thr 48,His 54,Ser 77,Ala 266,His 340 and Lys 384 are also highly conserved in the 6 goose isolatesThe corresponding residues in other NDV strains are commonly Met 48,Ser 54,Asn 77,Ile 266,Tyr 340 and Glu 384However,the sequences of receptor-binding related regions show no difference to the 14 reference strains from domestic or abroad

Sequence Analysis of Hemagglutinin-neuraminidase Gene of Newcastle Disease Virus Causing Clinical Disease in Geese