蛹虫草发酵液抗菌活性初步研究

对蛹虫草摇瓶发酵的乙酸乙酯和正丁醇萃取物进行了抗菌实验.结果表明,乙酸乙酯萃取物对细菌中的金黄色葡萄球菌、蜡状芽孢杆菌、变形杆菌、巨大芽孢杆菌、北京棒状杆菌和霉菌中的绿色木霉、黄曲霉有明显的抑菌作用;正丁醇萃取物对细菌中的马铃薯芽孢杆菌、变形杆菌、枯草芽孢杆菌、灵杆菌和霉菌中的绿色木霉以及黄曲霉有明显的抑菌作用,且提取物的抑菌作用随浓度增大而增强,而水相则没有抑菌活性.蛹虫草发酵液中具有抗菌活性物质.

Preliminary Study of the Anti-microbial Activities of Fermentation Broth of Cordyceps militaris

The broth of Cordyceps militaris fermentation in an oscillating manner were extracted with EtOAa and n-BuOH and the anti-microbial activity of the extracts were tested. The results showed that the extract by EtOAc presented a significant effect on Staphylococcus aureau,Bacillus cereus,Bacillus megateriurn,Proteus species,Corynebacterium pekinense,Trichoderma viride and Aspergillus flavus all of which belonged to bacteria ;the extract by n-BuOH presented significant inhibitory effect on Bacillus mesentericus, Bacillus subtilis, Proteus species, Bacterium prodigious, Trichoderma viride and Aspergillus flavus all of which belonged to bacterium;The inhibitory effect become stronger and stronger with the extract concentration increaseing and the water phase of the extract did not present any antimicrobial effects. As a result, the broth of Cordyceps militaris fermentation contained antimicrobial active substances.