Optimization of the fermentation of olive mill waste-waters by Aspergillus niger |
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Authors: | Moktar Hamdi Habib BouHamed Radhouane Ellouz |
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Institution: | (1) Centre de Biotechnologie de Sfax, BP-W, 3038 Sfax, Tunisia |
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Abstract: | Summary The fermentation of olive mill waste-waters (OMW) by Aspergillus niger was studied. On the basis of factorial design experiments, suspended solids and concentration of OMW, nitrogen source, sulphate and size of inocula were all found to be significant by affecting mycelium growth and chemical oxygen demand (COD) removal. Neither the absence of yeast extract, magnesium, sodium, potassium nor of calcium limited the growth of A. niger. With media lacking additional nitrogen and sulphate, the growth was limited. The optimal inoculum obtained was between 106 and 107 spores/g COD. The highest biomass and the greatest COD removal were obtained with removed COD to N:SO
inf4
sup¨-
ratios averaging 100 to 3:1.5.
Offprint requests to: M. Hamdi |
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