Short communication: Detection of non-culturable Vibrio cholerae O139, by PCR and fluorescent antibody methods,in laboratory microcosms |
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| Authors: | M S Islam M J Alam M A Miah A Felsenstein R B Sack |
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| Institution: | (1) Environmental Microbiology Laboratory, Laboratory Sciences Division, International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), GPO Box 128, 1000 Dhaka, Bangladesh |
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| Abstract: | Non-culturable Vibrio cholerae O139 was detected in microcosms by PCR and fluorescent-antibody (FA) techniques. When survival of V. cholerae O139 in microcosms was assessed by viable counting on culture media, the vibrio became non-culturable after 44 days and remained non-culturable for an additional 7 weeks. |
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| Keywords: | Microcosm non-culturable PCR Vibrio cholerae O139 |
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