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Effect of substrate stiffness on early human embryonic stem cell differentiation |
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| Authors: | Eroshenko Nikolai Ramachandran Rukmani Yadavalli Vamsi K Rao Raj R |
| Institution: | 1. Department of Bioengineering, University of California, Berkeley, CA, 94720, USA 2. QB3: California Institute for Quantitative Biological Research, University of California, Berkeley, CA, 94720, USA 3. Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA 4. Department of Electrical and Computer Engineering, Boston University, Boston, MA, 02215, USA |
| Abstract: | There is growing demand for robust DNA assembly strategies to quickly and accurately fabricate genetic circuits for synthetic biology. One application of this technology is reconstitution of multi-gene assemblies. Here, we integrate a new software tool chain with 2ab assembly and show that it is robust enough to generate 528 distinct composite parts with an error-free success rate of 96%. Finally, we discuss our findings in the context of its implications for biosafety and biosecurity. |
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