Selective expression of class II E alpha d gene in transgenic mice |
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Authors: | L C Burkly D Lo C Cowing R D Palmiter R L Brinster R A Flavell |
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Affiliation: | Biogen Research Corporation, Cambridge, MA 02142. |
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Abstract: | Class II genes of the MHC must be expressed by APC for activation of CD4+ T cells and efficient delivery of T cell help to B lymphocytes. Class II genes have restricted tissue expression and are under complex regulation. By using various deletion constructs of the class II E alpha d gene in transgenic mice we have mapped different 5' flanking regions which control E alpha d gene expression in distinct cell types. We demonstrate dissociate expression of E alpha d within the macrophage lineage as well as within the B cell lineage, and present evidence for a repressive element operative in B cells and macrophages. We describe the generation of novel transgenic lines with limited constitutive and inducible E alpha mRNA and I-E protein. |
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