| Fluorescent vital staining of plant sexual cell nuclei with DNA—specific fluorochromes and its application in gametoplast fusion |
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| 引用本文： | YANGHONGYUAN XINLIWU 等. Fluorescent vital staining of plant sexual cell nuclei with DNA—specific fluorochromes and its application in gametoplast fusion[J]. Cell research, 1993, 3(2): 121-130 |
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| 作者姓名： | YANGHONGYUAN XINLIWU 等 |
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| 作者单位： | [1]DepartmentofBiology,WuhanUniversity,Wuhan430072,China [2]DepartmentofBiology,WuhanUniversity,Wuhan430072 |
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| 摘 要： | DNA－binding fluorochromes are often used for vital staining of plant cell nuclei.However,it is not always sure whether the cells after staining still remain in living state.We chose several criteria to estimate the validity of real vital staining for sexual cell nuclei.These were:the cytoplasmic streaming in pollen tubes whose nuclei were stined,the simultaneous visualization of fluorochromatic reaction and nucleus staining in isolated generative cells,and the capability of isolated.prestained generative or sperm cells to fuse with other protoplasts.The results confirmed that 4,6-diamidino-2-phenylindole(DAPI),Hoechst 33258 and mithramycin could be used as real vital stains,though their efficiency varied from case to case;among them DAPI showed best effect.The fluo rescent vital staining technique offered a useful means foridentification and selection of heterokaryons in gametoplast manipulation studies.
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| 关 键 词： | 植物性细胞核 活细胞荧光染色 DNA特异性荧光染料 配子质体融合 应用 |
Fluorescent vital staining of plant sexual cell nuclei with DNA-specific fluorochromes and its application in gametoplast fusion |
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| YANG HONGYUAN,XINLI WU,YONGSHENG MO,CHANG ZHOU. Fluorescent vital staining of plant sexual cell nuclei with DNA-specific fluorochromes and its application in gametoplast fusion[J]. Cell research, 1993, 3(2): 121-130 |
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| Authors: | YANG HONGYUAN XINLI WU YONGSHENG MO CHANG ZHOU |
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| Affiliation: | YANG HONGYUAN,XINLI WU,YONGSHENG MO,CHANG ZHOUDepartment of Biology,Wuhan University,Wuhan 430072,China |
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| Abstract: | DNA-binding fluorochromes are often used for vital staining of plant cell nuclei. However, it is not always sure whether the cells after staining still remain in living state. We chose several criteria to estimate the validity of real vital staining for sexual cell nuclei. These were: the cytoplasmic streaming in pollen tubes whose nuclei were stained, the simultaneous visualization of fluo-rochromatic reaction and nucleus staining in isolated generative cells, and the capability of isolated, prestained generative or sperm cells to fuse with other protoplasts. The results confirmed that 4',6-diamidino-2-phenylindole (DAPI), Hoechst 33258 and mithramycin could be used as real vital stains, though their efficiency varied from case to case; among them DAPI showed best effect. The fluorescent vital staining technique offered a useful means fori-dentification and selection of heterokaryons in gametoplast manipulation studies. |
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| Keywords: | fluorescent vital staining DNA-specific fluorochrome generative cell sperm cell gametoplast fusion. |
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