Development of Polymorphic EST Markers Suitable for Genetic Linkage Mapping of Catfish |
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Authors: | Zhanjiang Liu Attila Karsi Rex A Dunham |
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Institution: | (1) The Fish Molecular Genetics and Biotechnology Laboratory, Department of Fisheries and Allied Aquacultures and Program of Cell and Molecular Biosciences, 203 Swingle Hall, Auburn University, Auburn, AL 36849, U.S.A., US |
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Abstract: | Expressed sequence tag (EST) markers are important for gene mapping and for marker-assisted selection (MAS). To develop EST
markers for use in catfish gene mapping, 100 randomly picked complementary DNAs from the channel catfish (Ictalurus punctatus) pituitary library were sequenced. The EST sequences were used to design primers to amplify channel catfish and blue catfish
(I. furcatus) genomic DNAs. Polymerase chain reaction products of the ESTs were analyzed to determine length polymorphism between the
channel catfish and blue catfish. Eleven polymorphic EST markers were identified. Five of the 11 EST markers were from known
genes and the other six were from unidentified ESTs. Seven ESTs were found to be associated with microsatellite sequences.
Analysis of channel catfish gene sequences indicated highly biased codon usage, with 16 codons being preferably used. These
codons were more preferably used in highly expressed ribosomal protein genes and in highly expressed pituitary hormone genes.
G/C-rich codons are less used in channel catfish than those in other vertebrates suggesting AT-richness of the channel catfish
genome.
Received June 29, 1998; accepted March 29, 1999. |
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Keywords: | : Expressed sequence tags catfish marker polymorphism gene mapping |
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