A high-performance liquid chromatography method for the quantification of cysmethynil,an inhibitor of isoprenylcysteine carboxylmethyl transferase,in mouse plasma |
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Authors: | Mei Wang Yok Moi Khoo Jin Zhou Patrick Casey How Sung Lee |
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Affiliation: | 1. Program of Cancer Stem Cell Biology, Duke-NUS Graduate Medical School, Singapore 169547, Singapore;2. Department of Pharmacology, National University of Singapore, Singapore 117597, Singapore |
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Abstract: | Cysmethynil, a newly identified small molecule inhibitor of isoprenylcysteine carboxylmethyl transferase (Icmt) is involved in the post-translational modification of CaaX proteins. Cysmethynil causes cell death in many human cancer cells in vitro, and inhibits tumor growth in the xenograft mouse model in vivo. A HPLC method for the quantification of cysmethynil in mouse plasma was developed and validated. The lower limit of quantification of this method was 0.01 μg/ml. Inter- and intra-day variability ranged from 0.38–8.5% and accuracy was between 86% and 98%. This sensitive method was used to quantify cysmethynil in plasma of mice after intraperitoneal dosing for preliminary pharmacokinetic studies. |
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