Development of a sensitive and selective LC/MS/MS method for the simultaneous determination of intracellular 1-beta-d-arabinofuranosylcytosine triphosphate (araCTP), cytidine triphosphate (CTP) and deoxycytidine triphosphate (dCTP) in a human follicular lymphoma cell line |
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| Authors: | Céline Crauste Isabelle Lefebvre Michael Hovaneissian Jean Yves Puy Béatrice Roy Suzanne Peyrottes Sabine Cohen Jérôme Guitton Charles Dumontet Christian Perigaud |
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| Institution: | 1. Institut des Biomolécules Max Mousseron, UMR 5247 CNRS-UM1-UM2, Université Montpellier 2, case courrier 1705, place Eugène Bataillon, 34095 Montpellier Cedex 05, France;2. Hospices Civils de Lyon, Laboratoire de pharmacologie, Hôpital E. Herriot, 5 Place d’Arsonval, 69437 Lyon, France;3. Hospices Civils de Lyon, Inserm, U590, Lyon F-69008, France |
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| Abstract: | A method was developed for the quantification of araCTP, CTP and dCTP in a human follicular lymphoma cell line. This method involves solid phase extraction (SPE) using a weak anion-exchanger (WAX) cartridge, a porous graphitic carbon high-performance liquid chromatography (HPLC) column separation, and tandem mass spectrometry (MS/MS) detection. By using a triple quadrupole mass spectrometer operating in negative ion multiple reaction monitoring (MRM) mode, the method was able to achieve a lower limit of quantification (LLOQ) of 0.1 μg mL?1 for araCTP and of 0.01 μg mL?1 for both CTP and dCTP. The method was validated and used to determine the amount of araCTP, CTP and dCTP formed after incubation of araC and an araCMP prodrug in the human follicular lymphoma cell line RL. |
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