Determination of in vivo disulfide-bonded proteins in Arabidopsis |
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| Authors: | Sophie Alvarez Gordon H Wilson Sixue Chen |
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| Institution: | 1. Department of Botany and Zoology, University of Florida, Gainesville, FL 32610, USA;2. Proteomics Facility, Interdisciplinary Center for Biotechnological Research, University of Florida, Gainesville, FL 32610, USA |
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| Abstract: | Protein thiol-disulfide oxidoreduction plays an important role in redox regulation of cellular processes. Here we present a proteomic approach to visualize and map in vivo disulfide-bonded proteins in plants. A proteomic map of the disulfide-bonded proteins was achieved using 2D gel electrophoresis of Arabidopsis protein extract. Along with novel proteins identified as potentially redox regulated, we have also shown the feasibility of mapping some of the cysteines involved in the formation of disulfide bonds. This study presents an important tool for characterizing redox-regulated proteins. |
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