Adaptive fuzzy control of nutrients concentration in fed-batch culture of mammalian cells |
| |
Authors: | Petros Lenas Tamotsu Kitade Hideki Watanabe Hiroyaki Honda Takeshi Kobayashi |
| |
Abstract: | An adaptive fuzzy controller was developed to control the glucose and glutamine concentrations in the reactor constant at
the desired level. The parameter values of the controller change during the cultivation according to the culture phase which
was detected by the lactate concentration. Cultivations with different glucose and glutamine set point concentrations of a
recombinant BHK anchorage-dependent cell line were performed in a fed-batch reactor on-line connected with an HPLC system.
Glucose and glutamine concentrations were satisfactorily controlled at each set point during all cultivation periods. Ammonia
had a determining effect on productivity since it inhibited cell growth and protein specific production. Ammonia production
increased with an increase of glutamine or a decrease of glucose set point concentrations, indicating the importance of glucose
to glutamine ratio for the optimization of productivity in mammalian cell cultures.
This revised version was published online in June 2006 with corrections to the Cover Date. |
| |
Keywords: | adaptive control ammonia antithrombin III BHK cells fed-batch culture fuzzy control HPLC |
本文献已被 PubMed SpringerLink 等数据库收录! |