A large inversion in the linear chromosome of <Emphasis Type="Italic">Streptomyces griseus</Emphasis> caused by replicative transposition of a new Tn<Emphasis Type="Italic">3</Emphasis> family transposon |
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Authors: | M Murata T Uchida Y Yang A Lezhava H Kinashi |
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Institution: | (1) Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan;(2) Present address: Omics Science Center, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan; |
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Abstract: | We have comprehensively analyzed the linear chromosomes of Streptomyces griseus mutants constructed and kept in our laboratory. During this study, macrorestriction analysis of AseI and DraI fragments of mutant 402-2 suggested a large chromosomal inversion. The junctions of chromosomal inversion were cloned and
sequenced and compared with the corresponding target sequences in the parent strain 2247. Consequently, a transposon-involved
mechanism was revealed. Namely, a transposon originally located at the left target site was replicatively transposed to the
right target site in an inverted direction, which generated a second copy and at the same time caused a 2.5-Mb chromosomal
inversion. The involved transposon named TnSGR was grouped into a new subfamily of the resolvase-encoding Tn3 family transposons based on its gene organization. At the end, terminal diversity of S. griseus chromosomes is discussed by comparing the sequences of strains 2247 and IFO13350. |
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