Isolation and characterization of a novel coenzyme Q from some methane-oxidizing bacteria |
| |
| Authors: | M D Collins P N Green |
| |
| Affiliation: | 1. Dpt. of Geo-environmental Processes and Global Change, Pyrenean Institute of Ecology — CSIC, Spain;2. Large Lakes Observatory and Department of Geological Sciences, University of Minnesota Duluth, Duluth, MN 55812, USA;3. Instituto de Geociencias (CSIC, UCM), Calle José Antonio Nováis, 2, 3ª planta, 3b, Facultad de Ciencias Geológicas, Univ. Complutense, 28040 Madrid, Spain;4. University of Pittsburgh, Geology and Planetary Science. 4107 O''Hara Street, Room 200, SRCC Building Pittsburgh, PA 15260-3332. USA;5. Institute of Earth Sciences Jaume Almera (ICTJA-CSIC), C/ Lluis Solé Sabaris s/n, Barcelona, E-08028 Spain;1. State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, China;2. Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of Forestry, China;3. Biotechnology Research Center of China Three Gorges University, Yichang, Hubei 443002, China;4. Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China |
| |
| Abstract: | The respiratory quinone composition of 18 strains of obligate methane-utilizing bacteria was examined. All of the strains contained lipoquinones which on examination by tlc co-chromatographed with coenzyme Q. On the basis of chromatographic and physicochemical analyses the lipoquinones produced by 10 of the strains corresponded to Q-8. Reverse-phase partition and argentation hplc demonstrated the quinone produced by the remaining 8 strains did not correspond to any known coenzyme Q prenologue. On the basis of mass spectrometry, 1H and 13C nuclear magnetic resonance spectrometry the novel quinone was shown to correspond to 2,3-dimethoxy-5-methyl-6-(18-methylene-3,7, 11,15,19,23,27,31-octamethyldotriacontahepta-2,6,10,14,22,26,30 enyl-)-1, 4-benzoquinone. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
