Resonance energy transfer study using a rhenium metal-ligand lipid conjugate as the donor in a model membrane. |
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Authors: | L Li I Gryczynski J R Lakowicz |
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Institution: | Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore 21201, USA. |
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Abstract: | We measured steady state and time-resolved resonance energy transfer between donors and acceptors in model membranes. The donor was a long lifetime rhenium-lipid complex, which displayed a mean lifetime of 1 microsecond and lifetime components as long as 3 microseconds in the labeled DOPC membranes. The transfer efficiencies were found to be substantially larger than those predicted without consideration of lateral diffusion. The larger transfer efficiencies are consistent with a mutual lateral diffusion coefficient in the membrane near 2 x 10(-8) cm2/s. These results demonstrate that lateral diffusion in membranes can be detected with microsecond lipid probes. |
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