Detection of a bromoperoxidase in Streptomyces phaeochromogenes |
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Authors: | K H van Pée F Lingens |
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Institution: | Laboratory for Physiological Chemistry, State University of Utrecht, Vondellaan 24 A, 3521 GG Utrecht, The Netherlands |
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Abstract: | Adenovirus DNA replication was studied in a partially reconstituted system consisting of purified viral proteins (DNA-binding protein, precursor terminal protein and Ad DNA polymerase) and a nuclear extract from uninfected HeLa cells. Optimal DNA replication required the presence of a heat-stable, ribonuclease-sensitive fraction from the cytosol of uninfected cells. This fraction stimulated the initiation about 3-fold and the replication of origin fragments 5-10-fold. Sedimentation analysis indicated the presence of a fast-sedimenting and a slow-sedimenting component which complemented each other. At least part of the stimulation was caused by low-molecular-mass RNA. |
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Keywords: | Adenovirus DNA replication in vitro Reconstitution Small RNA |
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