Biphasic response of human polymorphonuclear leucocytes and keratinocytes (epitheliocytes) fromXenopus laevis to mechanical stimulation |
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Authors: | W. Korohoda Monika Vöth J. Bereiter-Hahn |
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Affiliation: | (1) AK Kinematische Zellforschung, Johann Wolfgang Goethe Universität, Senckenberganlage 27, Postfach 11 19 32, D-W-6000 Frankfurt/M., Germany;(2) Present address: Institute for Molecular Biology, The Jan Zurzycki Institute, Jagiellonian University, Krakow, Poland |
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Abstract: | Summary Human polymorphonuclear leucocytes and epitheliocytes isolated from tadpole tails ofXenopus laevis were used to observe the responses of cells to mechanical stimulation with a microneedle. Biphasic responses were observed: a retraction phase lasting 1–3 s was followed by an extension phase lasting 10–40s. Weak stimulation evoked alocal response whilst on strong stimulation the whole cells rounded up. Spreading after induced rounding was at least one order of magnitude faster (it lasted less than 1–2min) than cell spreading after chemical dissociation of cell cultures. Local or extended loss of cell attachment to the substratum (observed with reflection interference contrast microscopy) preceded changes in cell morphology, visible with phase contrast microscopy. Repeated weak stimulation of one cell side induced extension and locomotion of the cell in this direction. The reported biphasic responses of cells to mechanical stimulation highlight the significance of exact timing when following any cell response to external stimuli. |
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Keywords: | Cell locomotion Mechanical stimulation Keratinocytes Polymorphonuclear leucocytes |
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