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| Cu2+对喜树愈伤细胞中喜树碱合成的影响 | |
| 引用本文： | 顾青,宋达峰,张虹,朱睦元. Cu2+对喜树愈伤细胞中喜树碱合成的影响[J]. 生物工程学报, 2006, 22(4): 624-628 |
| 作者姓名： | 顾青 宋达峰 张虹 朱睦元 |
| 作者单位： | 1. 浙江工商大学生物工程系,杭州,310035;浙江大学生命科学学院,杭州,310012 2. 浙江工商大学生物工程系,杭州,310035 3. 浙江大学生命科学学院,杭州,310012 |
| 摘 要： | 喜树碱是一种从木本植物喜树(Camptothecaacuminata)中分离得到的抗癌活性物质,通过细胞培养合成喜树碱是喜树碱生产的一条重要途径。研究Cu~(2 )对喜树愈伤细胞培养中喜树碱积累的影响,结果表明,在B5培养基中添加0.008mg/mLCuCl2时,对喜树愈伤细胞的生长没有明显影响,但是对喜树愈伤细胞合成喜树碱的促进作用最强,喜树碱含量比未诱导前增加了约30倍。Cu~(2 )阻止培养细胞后期过氧化物酶活力的下降,并抑制花青素的生成。与黑暗培养相比,光照条件下添加Cu~(2 )更利于喜树愈伤细胞诱导合成喜树碱。 |
| 关 键 词： | Cu~(2 ) 喜树 喜树碱 细胞培养 |
| 文章编号： | 1000-3061(2006)04-0624-05 |
| 收稿时间： | 2005-12-29 |
| 修稿时间： | 2006-03-31 |
Effects of Cu2+ on Biosynthesis of Camptothecin in Cell Cultures of Camptotheca acuminata |
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| GU Qing,SONG Da-Feng,ZHANG Hong,ZHU Mu-Yuan. Effects of Cu2+ on Biosynthesis of Camptothecin in Cell Cultures of Camptotheca acuminata[J]. Chinese journal of biotechnology, 2006, 22(4): 624-628 | |
| Authors: | GU Qing SONG Da-Feng ZHANG Hong ZHU Mu-Yuan |
| Affiliation: | 1 Department of Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, China; 2 State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310012, China |
| Abstract: | Camptothecin is a strong anti-tumor compound isolated from Camptotheca acuminata. One of the most important way for the production of Camptothecin is by cell cultures of Camptotheca acuminata. The effect of Cu~(2 ) on camptothecin accumulation in Camptotheca acuminata cell line was described in this paper. The results showed that the optimum CuCl-2 concentration in B5 medium was 0.008mg/mL, which increased camptothecin production for 30 times compare to the control while has no inhibitive effects on cell growth, at the same time, the peroxidase activity was increased and the anthocyanidin accumulation was inhibited. The promotive effects of Cu~(2 ) on camptothecin accumulation in light was higher than that in dark. |
| Keywords: | Cu~(2 ) Camptotheca acuminata CPT cell culture |
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