细胞外Mn^2＋对内向整流钾通道（IRK1）的阻断作用

目的和方法：采用双微电极电压钳（ＴＥＶ）法研究细胞外Ｍｎ＾２＋对非洲爪蟾卵母细胞表达的内向整流钾通道（ＩＲＫ１）的阻断作用。结果：细胞外Ｍｎ＾２＋浓度分别为１、１．２５、２．５、５、１０和２０ｍｍｏｌ／Ｌ，Ｋ＾＋浓度为９０ｍｍｏｌ／Ｌ，可见Ｍｎ＾２＋对ＩＲＫ１的瞬间电流（旋加电压后２ｍｓ）具有Ｍｎ＾２＋浓度依赖性和电压依赖性阻断作用；细胞外加Ｍｎ＾２＋浓度较高时强；细胞外Ｋ＾＋浓度为９０ｍｍｏｌ／

The blocking effects of extracellular Mn2+ on the inward rectifier potassium channel (IRK1)]

AIM AND METHODS: Two-microelectrode voltage clamp (TEV) method was used to study the blocking effects of extracellular Mn2+ on the inward rectifier potassium channel (IRK1) expressed in the Xenopus oocytes. RESULTS: Mn2+ can concentration-, time- and vol-tage dependently block IRK1 instantaneous currents (2 ms after voltage applied). Mn2+ has almost no effect on the gating property of IRK1. IRK1 can not permeate Mn2+ because reverse potential did not changed. External Mn2+ can inhibit IRK1 macroscopic currents more powerfully when external Mn2+ concentration is lower and external Mn2+ can increases standard chord conductance of IRK1. CONCLUSION: External Mn2+ works through surface potential mechanism. Ba2+ is considered as one fast open channel blocker of IRK1 and three exponential fitting results indicates that external Mn2+ can compete with Ba2+ in the same binding site in IRK1 when external Ba2+ concentration is 30 mumol/L. These mean two different mechanisms about external Mn2+ blocking exist.