Characterization of Two Novel cry8 Genes from Bacillus thuringiensis Strain BT185 |
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| Authors: | Changlong Shu Hong Yu Rongyan Wang Shuliang Fen Xudong Su Dafang Huang Jie Zhang Fuping Song |
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| Affiliation: | (1) Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China;(2) Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Beijing, China;(3) Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China |
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| Abstract: | Two novel cry8-type genes, cry8Ea1 and cry8Fa1, obtained from a Holotrichia parallela–specific Bacillus thuringiensis strain, BT185, were characterized. Findings showed that cry8Ea1 and cry8Fa1 encoded polypeptides of 1164 and 1174 amino acid residues, respectively. The deduced amino acid sequences of both Cry8Ea1 and Cry8Fa1 polypeptides are the most similar to that of Cry8Ba1. Eight conserved blocks (blocks 1–8) exist in Cry8Ea1 and Cry8Fa1 polypeptides compared with known Cry proteins. Cry8Ea1 and the Cry8Fa1 toxins could form spheric crystals when they were expressed in the acrystalliferous mutant strain HD73−. The spores and crystals from the recombinant strain containing cry8Ea1 were toxic to Holotrichia parallela, with an LC50 of 0.0875 × 108 colony-forming units (CFU)/g. However, Cry8Fa1 expressed in the recombinant strain was not toxic to H. parallela, Anomala corpulenta, or H. oblita. |
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