In vitro organotypic cultivation of adult newt and rat retinas |
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Authors: | Yu P Novikova K S Aleynikova M S Krasnov V A Poplinskaya E N Grygoryan |
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Institution: | 1.Koltzov Institute of Developmental Biology,Russian Academy of Sciences,Moscow,Russia |
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Abstract: | Adult rat and newt retinas were studied during long organotypic 3D cultivation. A high proliferation level was discovered
in the region of growth by applying DNA synthesis markers and in vitro mitosis registration in newt retina. Aggregates were
formed in the retina spheroid cavity because dedifferentiated cells migrated into this region. Small cell populations in nuclear
layers also had dividing and migration capacity. Rosette formation has been shown in newt retina. It is a characteristic of
fetal retinal development under pathological conditions. The antiGFAP antibody dye demonstrated an increase in the parent
Müller cell population and generation of a small cell pool with short GFAP-extensions de novo. Recoverin expression studies
detected its translocation from photoreceptor extensions to the cell bodies. Moreover, protein was presented in some cells
inside the spheroid. It has been shown for the first time that cell proliferation occurred in the adult rat retinal spheroid
developing in vitro; BrdU-positive cells and multiple mitoses were revealed in this fissue. However, the source of proliferation
was not in the peripheral retina, and resident macrophages and glial cells located among neurons of the inner nuclear layer
had the ability to divide. The antiGFAP antibody showed an increase in GFAP fibers in the rat retina as well as in the newt
retina. Recoverin translocated into photoreceptor perikaryons and the outer plexiform layer in cultivated rat retina. Interestingly,
some cells with probably de novo expression of recoverin were discovered in rat and newt inner retinas. |
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