The structure of sterile cytoplasm types within a maize genebank collection |
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Authors: | J Van?etovi? M Vidakovi? D Ignjatovi?-Mici? A Nikoli? K Markovi? V An?elkovi? |
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Institution: | (1) Laboratory of Genetic Resources & Functional Improvement for Horticultural Plants, Department of Horticulture, Zhejiang University, Hangzhou, 310029, P.R. China;(2) Laboratory of Horticultural Plant Growth, Development & Biotechnology, Ministry of Agriculture, Hangzhou, 310029, P.R. China; |
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Abstract: | Maize Research Institute (MRI) gene bank maintains a collection of 6000 maize accessions. Within this collection over 100
sources of cytoplasmic male sterility (CMS) were found in field trials, i.e. more than 2% of the total accession numbers.
These sources are distributed among Yugoslav open-pollinated varieties (4.56% of them contain CMS), as well as introduced
heterozygous genotypes and inbred lines. In order to identify cytoplasm types the gene-bank sources of CMS were screened using
a PCR assay with specific primers for C, T and S cytoplasms. Predominant cytoplasmic male sterility type among the analyzed
accessions was CMS-S. Results were inconclusive for three accessions, i.e. different results for the progenies of two ears
per accession were obtained. For another two accessions a new PCR product profile was identified, consisting of one band characteristic
for CMS-S and one unspecific for any of the three CMS types. The PCR approach enabled a simple, fast and reliable large scale
screening of maize cytoplasm among MRI gene bank accessions, significantly reducing time for cytoplasm characterizations compared
to classical method of testing with restorers for each known type of CMS. |
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