Effect of hypoxia on the antioxidative enzymes in aerobically grown rice (Oryza sativa) seedlings |
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| Authors: | Takashi Ushimarua Sumio Kanematsub Mineo Shibasakac Hideo Tsujid |
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| Affiliation: | Department of Biology and Geoscience, Faculty of Science, Shizuoka University, Shizuoka 422-8529, Japan; Department of Food Technology, Faculty of Horticulture, Minamikyushu University, Miyazaki 884-0003, Japan; Research Institute for Bioresources, Okayama University, Kurashiki 710-0046, Japan; Department of Biology, Kobe Women's University, Kobe 654-8585, Japan |
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| Abstract: | The development of the antioxidative system is repressed in rice ( Oryza sativa L. cv. Yamabiko) seedlings germinated under water, but the system is synthesized within 24 h after exposure of the seedlings to air. In order to see whether this regulation of the antioxidative system is reversible, the changes were investigated after submergence of aerobically grown seedlings under water. Low survivability, no morphological change and a slight increase in activity of alcohol dehydrogenase (ADH; EC 1.1.1.1) were observed 24 h after the submergence. Activities of some antioxidative enzymes were decreased, but they were still higher than those in seedlings germinated under water. Superoxide dismutase (SOD; EC 1.15.1.1) in mitochondria, in addition to cytosolic forms of SOD, showed no significant changes, whereas plastidic SOD decreased drastically, which reflected the changes in the development of the corresponding organelles. Thus, the regulation of the antioxidative system was, in part, reversible. In contrast, catalase (EC 1.11.1.6) activity alone was increased by the shift from air to submergence. |
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