Comparison of Two Superfusion Systems for Study of Neurotransmitter Release from Rat Cerebral Cortex Slices |
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| Authors: | E. R. Korpi S. S. Oja |
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| Affiliation: | Department of Biomedical Sciences, University of Tampere, Tampere, Finland |
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| Abstract: | Depolarization-induced release of [3H]gamma-aminobutyric acid (GABA) and [3H]noradrenaline (NA) from rat cerebral cortex slices was studied in two superfusion systems: one with stationary and the other one with continuously shaken slice compartments. Calcium-dependent depolarization-induced release of GABA and NA could be demonstrated only with shaken slices. GABA, but not NA, could also be released by high K+ media and veratridine from stationary slices. Synaptic transmitter releasing mechanisms are apparently damaged in stationary slices, possibly due to impaired energy metabolism. |
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| Keywords: | Neurotransmitter release Superfusion of brain slices Maintenance of synaptic mechanisms |
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