pUB307 mobilizes resistance plasmids from Escherichia coli into Neisseria gonorrhoeae |
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| Authors: | Jean-Claude Piffaretti Achille Arini Joachim Frey |
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| Affiliation: | (1) Istituto Cantonale Batteriologico, via Ospedale 6, CH-6904 Lugano, Switzerland;(2) Département de Biochimie, Sciences II, Quai Ernest-Ansermet 30, CH-1211 Genève 4, Switzerland;(3) Present address: Department of Microbiology and Immunology, Emory University School of Medicine, 30322 Atlanta, GA, USA;(4) Present address: Veterinär-Bakteriologisches Institut der Universität, CH-3012 Bern, Switzerland |
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| Abstract: | Summary The plasmid pUB307, a derivative of RP1, is a conjugative, broad-host-range plasmid. We have shown that this element mobilizes gonococcal resistance plasmids from Escherichia coli to Neisseria gonorrhoeae, thus providing evidence that extrachromosomal elements can efficiently enter gonococci by conjugation. Furthermore, pUB307 can also be used as a helper element to mobilize the cloning vector pLES2 into N. gonorrhoeae. This finding significantly increases the usefulness of pLES2 as a shuttle vector between E. coli and gonococcus. |
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| Keywords: | Neisseria gonorrhoeae IncP plasmids Mobilization Conjugation Cloning vector |
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