大菜粉蝶颗粒体病毒的形态发生及细胞病理的超微结构观察

本文叙述感染大菜粉蝶颗粒体病毒后,病虫脂肪体细胞超微结构的改变,大菜粉蝶感染后24小时,病虫脂肪体细胞开始出现明显的病变,整个病程是,在开始时细胞核内出现清晰区并出现病毒发生基质,核膜多点成套增生,其后核膜断裂,大量膜样结构聚集在病毒发生基质的周围,核衣壳大量产生,有一部分核衣壳从这些病毒发生基质四周的膜样结构碎片上获得套膜,荚膜蛋白沉积形成成熟的病毒荚膜,或称包含体;另一部分则排列在胞浆内的空泡边缘上;其余的核衣壳则从细胞边缘“芽突”而获得套膜,另外还描述环孔片层及线粒体改变。

PIERIS BRASSICAE GRANULOSIS VIRUS MORPHOGENESIS AND ULTRASTRUCTURAL CYTOPATHOLOGY OF INFECTED FAT BODY CELLS

The present study primarily details some changes accompaning the virus infection in larval fat body cells. 24 hours after infection, cytopatho-iogical alteration appeared. At early stage of infection, the remainder of the nucleus contained only small remains of hetrochromatin resulting in a "cleared" area, and the virogenic stromata appeared, nuclear membrane duplicated and reduplicated. After nuclear membranes broke down, the virogenic stromata were surrounded by membrane-like structures, and nucleo- capsids produced. Some nucleocapsids aquired viral envelope from the membranous fragment surrounding these virogenic stromata, then embedded in capsule protein to form mature capsule called inclusion body. Part of cap-sidss arranged along the vacuqlar membrane in cytoplasm. The other nucleocapsids budded from the infected cells and acquired envelopes as a result. We assume that nucleocapsids may be released by these vacuoles without any damages o,f the cell and may be infectious. Annulate lamellae were found in infected cells during the whole developing process of the virus. Nucleolus-like material existed in an advanced stage of infection. The outer and inner membranes area of mitochondria increased and cristal membrane duplicated just like the nuclear membrane. Some mitochodria were dividing.