Brain 12-HETE formation in different species,brain regions,and in brain microvessels |
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| Authors: | Gary S Hambrecht Sunday A Adesuyi Sallie Holt Earl F Ellis |
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| Institution: | (1) Department of Pharmacology and Toxicology, Medical College of Virginia, Virginia Commonwealth Univesity, Richmond, Virginia, USA;(2) Present address: Nova Pharmaceutical Corp., 5210 Eastern Ave., 21224 Baltimore, MD;(3) Present address: Department of Mathematics and Science, St. Paul's College, 23868 Lawrenceville, VA;(4) MCV Station, Box 613, 23298 Richmond, VA |
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| Abstract: | We used gas chromatography/mass spectrometry to measure brain 12-HETE (12-Hydroxy-5,8,10,14-eicosatetraenoic acid) formation from endogenous arachidonic acid in different species and different brain regions and in isolated brain microvessels. When blood-free brain slices were incubated for 20 minutes we found that the rabbit and cat brain incubates contained little 12-HETE when compared to rat and mouse brain incubates. Further in vitro studies of various rat brain regions showed a generally even distribution of 12-HETE. When isolated rat or rabbit microvessels were incubated and analyzed, we found 1 and 0.25  g, respectively, of 12-HETE/mg of microvessel protein. Also, rabbit brain had limited or no capacity to actively metabolize tritiated 12-HETE. In summary, these studies show substantial species variation with respect to brain formation of 12-HETE and indicate that the vasculature is a potentially significant contributor to the 12-HETE found in whole brain tissue. |
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| Keywords: | Lipoxygenase brain microvessels 12-HETE arachidonic acid |
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