Chloroplast genome aberration in micropropagation-derived albino <Emphasis Type="Italic">Bambusa edulis</Emphasis> mutants, <Emphasis Type="Italic">ab1</Emphasis> and <Emphasis Type="Italic">ab2</Emphasis> |
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Authors: | Nien-Tsu Liu Wann-Neng Jane Hsin-Sheng Tsay Hui Wu Wei-Chin Chang Choun-Sea Lin |
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Institution: | (1) Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China;(2) Institute of Biotechnology, Chaoyang University of Technology, Taichung, Taiwan, Republic of China;(3) Department of Biotechnology, China Institute of Technology, 222 4F, Mingseng E. RD. 5 sec, Taipei, Taiwan, Republic of China |
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Abstract: | Two albino mutants (ab1 and ab2) have been derived from long-term shoot proliferation of Bambusa edulis. Based on transmission electronic microscopy data, the chloroplasts of these mutants were abnormal. To study the mutation
of gene regulation in the aberrant chloroplasts, we designed 19 pairs of chloroplast-encoded gene primers for genomic and
RT-PCR. Only putative NAD(P)H-quinone oxidoreductase chain 4L (ndhE; DQ908943) and ribosomal protein S7 (rps7; DQ908931) were conserved in both the mutant and wild-type plants. The deletions in the chloroplast genome of these two mutants
were different: nine genes were deleted in the chloroplast genomic aberration in ab1 and 11 genes in ab2. The chloroplast genes, NAD(P)H-quinone oxidoreductase chain 4 (ndhD; DQ908944), chloroplast 50S ribosomal protein L14 (rpl14; DQ908934), and ATP synthase beta chain (atpB; DQ908948) were abnormal in both mutants. The gene expressions of 18 of these 20 genes were correlated with their DNA copy
number. The two exceptions were: ATP synthase CF0 A chain (atpI; DQ908946), whose expression in both mutants was not reduced even though the copy number was reduced; ribosomal protein S19 (rps19; DQ908949), whose expression was reduced or it was not expressed at all even though there was no difference in genomic copy
number between the wild-type and mutant plants. The genomic PCR results showed that chloroplast genome aberrations do occur
in multiple shoot proliferation, and this phenomenon may be involved in the generation of albino mutants. |
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Keywords: | Albino mutant Bamboo Chloroplast genome |
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