|
|||||
|
|
Rice ternary MADS protein complexes containing class B MADS heterodimer |
|
| Authors: | Seok Hye-Yeon Park Hee-Yeon Park Ji-Im Lee Young-Mi Lee Sun-Young An Gynheung Moon Yong-Hwan |
| Institution: | a Department of Molecular Biology, Pusan National University, Busan 609-735, Republic of Korea b Ulsan University Hospital Biomedical Research Center, University of Ulsan, Ulsan 680-749, Republic of Korea c Department of Plant Molecular Systems Biotechnology, Crop Biotechnology Institute, Kyung Hee University, Yongin 446-701, Republic of Korea |
| Abstract: | To investigate ternary MADS protein complexes involved in the regulation of floral organ development in rice, we identified MADS proteins interacting with the class B MADS heterodimers, OsMADS16-OsMADS4 and OsMADS16-OsMADS2, using yeast three-hybrid assay. The class B heterodimers interacted with OsMADS6, 7, 8, 14 and 17, which belong to AP1-like, SEP-like or AGL6-like MADS proteins, generating ternary complexes. The entire region of the K and C domains of OsMADS4 was required for the formation of the OsMADS16-OsMADS4-OsMADS6 and OsMADS16-OsMADS4-OsMADS7 ternary complexes. Analysis results of transgenic plants concomitantly suppressing OsMADS4 and OsMADS6, together with the results of previous studies, suggest that the OsMADS16-OsMADS4-OsMADS6 ternary complex plays an important role in floral development, especially lodicule development. |
| Keywords: | C carboxyl-terminal I intervening K K-box M MADS-box Os Oryza sativa |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |