Effect in vitro of apolipoprotein A-V on the structure and functions of recombinant high density lipoprotein |
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Authors: | Xinbo Zhang |
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Affiliation: | National Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China |
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Abstract: | The neighboring position of apolipoprotein A-I (apoA-I) and apolipoprotein A-V (apoA-V) gene and the modulation of apoA-V on the concentrations, size and maturation of high density lipoprotein (HDL) may indicate a special relationship between apoA-V and HDL. To assess the effects of apoA-V on HDL structure and related functions in vitro, a series of recombinant HDL (rHDL) were synthesized in vitro with various mass ratios of recombinant apoA-I: apoA-V. An increase in apoA-V in rHDL resulted in enhanced lipid-binding ability, increased phospholipid content and larger particle size. Furthermore, the lipid-free and lipid-bound apoA-V in rHDL showed antioxidant capacity against low density lipoprotein (LDL) in vitro. In THP-1 derived macrophages, apoA-V of rHDL was shown to have no influence on the uptake of oxidized LDL (oxLDL) and intracellular lipid accumulation. Thus, the addition of apoA-V to rHDL resulted in changes in several rHDL properties, including increased lipid-binding ability, phospholipid content, particle size and antioxidant capacity. These alterations may explain the modulation of apoA-V on HDL in vivo and the beneficial functions of apoA-V on atherosclerosis. |
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Keywords: | ApoA-I, apolipoprotein A-I ApoA-V, apolipoprotein A-V DMPC, 1,2-dimyristoyl-sn-glycerol-3-phosphatidylcholine HDL, high density lipoprotein LCAT, lecithin:cholesterol acyltransferase MDA, malondialdehyde oxLDL, oxidized LDL rHDL, recombinant high density lipoprotein SPC, soybean lecithin TC, total cholesterol TG, triglyceride VLDL, very low density lipoprotein |
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