FOXO1 is a direct target of EWS-Fli1 oncogenic fusion protein in Ewing's sarcoma cells |
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Authors: | Yang Liu Hu Hsien-Ming Zielinska-Kwiatkowska Anna Chansky Howard A |
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Affiliation: | Department of Orthopedics, University of Washington, Seattle, WA 98195, United States Medical Research Service, VA Puget Sound Health Care System, Seattle, WA 98108, United States |
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Abstract: | Ewing’s family tumors are characterized by a specific t(11;22) chromosomal translocation that results in the formation of EWS-Fli1 oncogenic fusion protein. To investigate the effects of EWS-Fli1 on gene expression, we carried out DNA microarray analysis after specific knockdown of EWS-Fli1 through transfection of synthetic siRNAs. EWS-Fli1 knockdown increased expression of genes such as DKK1 and p57 that are known to be repressed by EWS-Fli1 fusion protein. Among other potential EWS-Fli1 targets identified by our microarray analysis, we have focused on the FOXO1 gene since it encodes a potential tumor suppressor and has not been previously reported in Ewing’s cells. To better understand how EWS-Fli1 affects FOXO1 expression, we have established a doxycycline-inducible siRNA system to achieve stable and reversible knockdown of EWS-Fli1 in Ewing’s sarcoma cells. Here we show that FOXO1 expression in Ewing’s cells has an inverse relationship with EWS-Fli1 protein level, and FOXO1 promoter activity is increased after doxycycline-induced EWS-Fli1 knockdown. In addition, we have found that direct binding of EWS-Fli1 to FOXO1 promoter is attenuated after doxycycline-induced siRNA knockdown of the fusion protein. Together, these results suggest that suppression of FOXO1 function by EWS-Fli1 fusion protein may contribute to cellular transformation in Ewing’s family tumors. |
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Keywords: | Ewing&rsquo s sarcoma EWS-Fli1 Oncogenic fusion protein FOXO1 RNA interference Inducible siRNA Tumor suppressor gene |
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