Th1 cells promote neurite outgrowth from cortical neurons via a mechanism dependent on semaphorins |
| |
Authors: | Ishii Hiroshi Kubo Takekazu Kumanogoh Atsushi Yamashita Toshihide |
| |
Institution: | a Department of Molecular Neuroscience, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan b Department of Neurobiology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuoku, Chiba 260-8677, Japan c Department of Immunopathology, Research Institute for Microbial Diseases, and World Premier International Immunology Frontier Research Center, Osaka University, Suita, Osaka 565-0871, Japan d JST, CREST, 5, Sanbancho, Chiyodaku, Tokyo 102-0075, Japan |
| |
Abstract: | The roles of T lymphocytes in the central nervous system (CNS) are diverse; their roles in the injured CNS have been reported to be both detrimental and advantageous. Hence, an investigation of the effects of specific subsets of T cells on neurons may provide an insight into the interaction between the nervous system and the immune system. In the present study, we demonstrate that a specific subset of T lymphocytes enhanced neurite outgrowth in vitro. When cultured T helper type 1 (Th1) cells were co-cultured with cortical neurons, neurite outgrowth from neurons was enhanced; however, the same was not observed when Th2 or naïve T cells were used. We observed that the promotion of neurite outgrowth by Th1 cells was completely inhibited by anti-interferon γ (IFN-γ) neutralizing antibody, but that IFN-γ did not directly promote neurite growth. Furthermore, experiments using knockout mice revealed that semaphorin 4A (Sema4A) but not Sema7A was required for the effect produced by Th1 cells. These results demonstrate that Sema4A and IFN-γ expressed in Th1 cells play a critical role in enhancing neurite outgrowth from cortical neurons. |
| |
Keywords: | CNS central nervous system Th1 T helper type 1 IFN-γ interferon γ Sema4A semaphorin 4A IL interleukin EAE experimental autoimmune encephalomyelitis FBS fetal bovine serum PBS phosphate-buffered saline FACS fluorescence-activated cell sorting BSA bovine serum albumin FITC fluorescein isothiocyanate APC allophycocyanin |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|