Isolation and purification of d-mannose from palm kernel |
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Authors: | Tao Zhang Ziguo Pan Chao Qian Xinzhi Chen |
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Affiliation: | aDepartment of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, People’s Republic of China |
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Abstract: | An economically viable procedure for the isolation and purification of d-mannose from palm kernel was developed in this research. The palm kernel was catalytically hydrolyzed with sulfuric acid at 100 °C and then fermented by mannan-degrading enzymes. The solution after fermentation underwent filtration in a silica gel column, desalination by ion-exchange resin, and crystallization in ethanol to produce pure d-mannose in a total yield of 48.4% (based on the weight of the palm kernel). Different enzymes were investigated, and the results indicated that endo-β-mannanase was the best enzyme to promote the hydrolysis of the oligosaccharides isolated from the palm kernel. The pure d-mannose sample was characterized by FTIR, 1H NMR, and 13C NMR spectra. |
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Keywords: | smCaps" >d-Mannose Endo-β -mannanase Hydrolysis Palm kernel |
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